viability dye live/dead® fixable aqua dead cell stain #l34966 (Thermo Fisher)

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Thermo Fisher viability dye live/dead® fixable aqua dead cell stain #l34966
Viability Dye Live/Dead® Fixable Aqua Dead Cell Stain #L34966, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aqua+fixable+viability+dye/live+dead+fixable+aqua+dead+cell+stain+kit/pm40659125-169-9-18
Average 90 stars, based on 1 article reviews

viability dye live/dead® fixable aqua dead cell stain #l34966 - by Bioz Stars, 2026-09

90/100 stars

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Incubation:

Article Title: PemBla: A Phase 1 study of intravesical pembrolizumab in recurrent non‐muscle‐invasive bladder cancer
Article Snippet: Live frozen peripheral blood mononuclear cells (PBMCs) were thawed and washed with Roswell Park Memorial Institute (RPMI) supplemented with 10% FBS and 12.5 U/ml Benzonase (Merck). .. Two million PBMCs of each sample were washed with PBS and incubated with Aqua Fixable viability dye (ThermoFisher Scientific) for 10 min at room temperature in the dark. ..

Article Title: Effects of Fish Oil on HIV-Related Inflammation and Markers of Immunosenescence: A Randomized Clinical Trial
Article Snippet: .. The next morning, the cells were washed and resuspended in 1 mL of phosphate-buffered saline (PBS), and incubated for 30 min in the dark at room temperature (RT) with 1 μL/test of AQUA fixable viability dye (LifeTechnologies, Carlsbad, CA). .. Cells were washed and resuspended in fluorescence-activated cell sorting (FACS) buffer (0.2% bovine serum albumin +0.1% NaN 3 in PBS) with 10 μL/test of Fc receptor block (Miltenyi Biotec, Inc., Auburn, CA) for 30 min at RT in the dark then washed in FACS buffer and incubated with PerCP Cy5.5 antihuman-CD8a and APC antihuman-CD4 (Tonbo Biosciences, San Diego, CA), PE Cy-7 antihuman CD28 and PB antihuman CD57 (BioLegend, San Diego, CA) for 30 min at 4°C in the dark.

Article Title: PemBla: A Phase 1 study of intravesical pembrolizumab in recurrent non‐muscle‐invasive bladder cancer
Article Snippet: Tumours and normal biopsies were dissociated mechanically and enzymatically using a tumour dissociation kit (Miltenyi Biotec 130‐095‐929). .. Samples were washed with PBS and incubated with Aqua Fixable viability dye (ThermoFisher Scientific) for 10 min at room temperature in the dark. ..

other:

Article Title: Spatiotemporal immune atlas of the first clinical-grade, gene-edited pig-to-human kidney xenotransplant
Article Snippet: Cells were washed with 45 mL of PBS and then stained with 1 μL/1×10 cells Aqua fixable viability dye (Invitrogen Life Scientific TM of ThermoFisher Scientific; Eugene, OR).

Article Title: Spatiotemporal immune atlas of the first clinical-grade, gene-edited pig-to-human kidney xenotransplant
Article Snippet: Cells were washed with 45 mL of PBS and then stained with 1 μL/1x10 cells6 Aqua 282 fixable viability dye (Invitrogen Life ScientificTM of ThermoFisher Scientific; Eugene, OR).

Saline:

Staining:

Article Title: Genetic Ablation of HLA Class I, Class II, and the T-cell Receptor Enables Allogeneic T Cells to Be Used for Adoptive T-cell Therapy
Article Snippet: 4 Department of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 1A8 13 * These authors contributed equally to this work.. 14 Running title: HLA class I/II and TCR ablation in antitumor allo-T cells 15 16

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